Welcome to Hiroaki Oda's homepage [Japanese]
Lab. Nutritional Biochemistry, Nagoya University, Nagoya 464-01, Japan
Phone: +81-52-789-4124Fax: +81-52-789-5050
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Lab. Nutr. Biochem. | School of Agricultureal Sciences | Nagoya University | Nagoya University COOP (Japanese)
How can hepatocytes keep hepatocyte-phenotype?
Liver, a main tissue for drug metabolism, carbohydrate metabolism and lipid metabolism, is highly differentiated. Although several liver-enriched transcription factors have been cloned, none of the transcription factors so far characterized is able to trigger the liver differentiation program in non-liver cells. We have found that the primary cultured hepatocyte is good model for investigating liver-differentiation. Monolayer hepatocytes cultured under usual conditions lose liver-specific functions after plating. However, hepatocytes cultured on laminin-rich basement membrane gel show spherical shapes and keep high levels of liver-specific functions. Some liver-enriched transcription factors have been identified to be involved in the maintenance of liver-specific functions in the hepatocyte culture. Although one of our final goals is to construct an artificial liver preparation, we are now focusing our efforts on 1) cloning of novel liver-specific transcription factors, 2) determination of cis-acting elements for extracellular matrix signals and cell shape, and 3) identification of signal transduction pathways from extracellular matrix to nucleus.
Freshwater Clam Extract Ameliorates Triglyceride and Cholesterol Metabolism through the Expression of Genes Involved in Hepatic Lipogenesis and Cholesterol Degradation in Rats
Thomas Laurent ,ﾊYuji Okuda ,ﾊTakeshi Chijimatsu ,ﾊMiki Umeki ,ﾊSatoru Kobayashi ,ﾊYutaro Kataoka ,ﾊIwao Tatsuguchi ,ﾊSatoshi Mochizuki ,ﾊand Hiroaki Oda
Evidence-Based Complementary and Alternative Medicine
Volumeﾊ2013ﾊ(2013), Article IDﾊ830684, 10 pages
Lipid components prepared from a freshwater clam (Corbicula fluminea) extract ameliorate hypercholesterolemia in rats fed high-cholesterol diet
Takeshi Chijimatsu, Miki Umeki, Yutaro Kataoka, Satoru Kobayashi, Koji Yamada, Hiroaki Oda and Satoshi Mochizuki
Food Chem. 136, 328-334 (2013)
Spherical cell shape of FLC-4 cell, a human hepatoma cell, enhances hepatocyte-specific function and suppresses tumor phenotype through the integration of mRNA-microRNA interaction
Thomas Laurent, Yutaro Kataoka, Satoru Kobayashi, Misaki Ando, Seishi Nagamori and Hiroaki Oda
Biol. Open 1, 958-964 (2012)
Real-time monitoring in three-dimensional hepatocytes reveals that insulin acts as a synchronizer for liver clock
Daisuke Yamajuku, Takahiro Inagaki, Tomonori Haruma, Shingo Okubo, Yutaro Kataoka, Satoru Kobayashi, Keisuke Ikegami, Thomas Laurent, Tomoko Kojima, Keiji Noutomi, Seiichi Hashimoto and Hiroaki Oda
Sci. Rep. 2, 439; DOI:10.1038/srep00439 (2012)
A novel human hepatoma cell line, FLC-4, exhibits highly enhanced liver differentiation functions through the three-dimensional cell shape
Thomas Laurent, Daiki Murase, Sayaka Tsukioka, Tomokazu Matsuura, Seishi Nagamori and Hiroaki Oda
J. Cell. Physiol. 227, 2898-2906 (2012)
J. Cell. Physiol.
Highlights: Volume 227, Number 7 (pages fm iv-fm v)
Application of a New Genotoxicity Test System with Human Hepatocyte Cell Lines to Improve the Risk Assessment in the Drug Development
Hashizume, T. and Oda, H.
Toxicity and Drug Testing, William Acree (Ed.), ISBN: 978-953-51-0004-1, InTech (2012)
Photographs of liver and cultured hepatocytes
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8144 Updated 4/28/13